a simplified van erth single nucleotide polymorphism (snp) typing method of bacillus anthracis applicable by traditional thermocycler machines

نویسندگان

زهرا نجفی علیا

najafi olya, z. (bsc) department of veterinary aerobic bacteria, razi institute, karaj,iranبخش تحقیق و تولید واکسن های باکتریایی هوازی دامپزشکی کیوان تدین

tadayon, k. (phd) department of veterinary aerobic bacteria, razi institute, karaj, iranبخش تحقیق و تولید واکسن های باکتریایی هوازی دامپزشکی رایناک قادری

ghaderi, r. (bsc) razi institute, karaj 3197619751, iranبخش تحقیق و تولید واکسن های باکتریایی هوازی دامپزشکی

چکیده

abstract snp typing is now a well-established genotyping system in bacillus anthracis studies. in the original standard method of van erth, snps at 13 loci of the b. anthracis genome were analyzed. in order to simplify and make appropriate this expensive method to low-budget laboratory settings, 13 primer pairs targeting the 13 corresponding snps were designed. besides, a universal pcr protocol was developed to enable simultaneous amplification of all loci by conventional pcr machines. the efficiency of this approach was approved by applying on nine isolates of b. anthracis. we recommend using this modified procedure as an efficient alternative to van erth method until developing newer and affordable techniques. keywords: bacillus anthracis, genotyping, snps, pcr

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A Simplified Van Erth Single Nucleotide Polymorphism (SNP) Typing Method of Bacillus Anthracis Applicable by Traditional Thermocycler Machines

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medical laboratory journal

جلد ۹، شماره ۱، صفحات ۹۷-۱۰۳

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